Expanding capabilities and infrastructure for cryo-EM
نویسندگان
چکیده
منابع مشابه
Persistent topology for cryo-EM data analysis.
In this work, we introduce persistent homology for the analysis of cryo-electron microscopy (cryo-EM) density maps. We identify the topological fingerprint or topological signature of noise, which is widespread in cryo-EM data. For low signal-to-noise ratio (SNR) volumetric data, intrinsic topological features of biomolecular structures are indistinguishable from noise. To remove noise, we empl...
متن کاملCryo-EM of viruses and vaccine design.
Knowledge of the atomic resolution structures of viruses can be a powerful tool for vaccine discovery and design. X-ray crystallography has long served as an invaluable method for virus structure determination at high resolution, but over the past decade, cryoelectron microscopy (cryo-EM) has begun to emerge as a complementary method that can also provide this kind of information. Crystallograp...
متن کاملCryo-EM enters a new era
Advances in detector hardware and image-processing software have led to a revolution in the use of electron cryo-microscopy to determine complex molecular structures at high resolution.
متن کاملTools for the cryo-EM gold rush: going from the cryo-EM map to the atomistic model
As cryo-electron microscopy (cryo-EM) enters mainstream structural biology, the demand for fitting methods is high. Here, we review existing flexible fitting methods for cryo-EM. We discuss their importance, potential concerns and assessment strategies. We aim to give readers concrete descriptions of cryo-EM flexible fitting methods with corresponding examples.
متن کاملCryo-EM structure of a transcribing cypovirus.
Double-stranded RNA viruses in the family Reoviridae are capable of transcribing and capping nascent mRNA within an icosahedral viral capsid that remains intact throughout repeated transcription cycles. However, how the highly coordinated mRNA transcription and capping process is facilitated by viral capsid proteins is still unknown. Cypovirus provides a good model system for studying the mRNA ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Methods
سال: 2021
ISSN: 1548-7091,1548-7105
DOI: 10.1038/s41592-021-01139-x